5 Maintenance
Installing or removing the nebulizer
Agilent Triple Quadrupole LC/MS System User Guide 108
4 Loosen the locknut next to the zero-dead-volume (ZDV) union by placing the
5/16-inch wrench on the nut, and the 1/4-inch wrench on the union, and
turning the 5/16-inch wrench clockwise.
5 Remove the union from the nebulizer needle holder.
6 Remove the union lock nut from the nebulizer.
7 Loosen the fastener (locknut, 1.5-mm hex screw or T6 Torx screw) that
secures the needle holder in place.
8 Loosen the needle holder and pull it out of the nebulizer body. Dispose of the
used needle properly.
9 For more thorough cleaning, sonicate the nebulizer body. Pour isopropanol or
methanol through the inside of the shaft.
10 Thread the new needle holder into the nebulizer body.
11 Carefully remove the new needle from the shipping tube and adhesive strip.
12 Identify the sharp end of the needle.
13 Carefully slide the sharp end of the needle through the ferrule until 1 cm of the
needle remains at the ferrule.
Do not bend the needle as you slide it into the ferrule. The needle is fragile. A
bent needle results in poor alignment with the nebulizer body.
14 Push the needle into the ferrule until the needle is slightly visible flush with the
ferrule.
15 Reinstall the locknut and the union. Hand-tighten the union.
16 Hold the needle holder steady with a 1/4-inch x 5/16-inch open-end wrench,
depending on your nebulizer holder style.
17 Tighten the union one-half to three quarters of a turn, or until an audible
squeak is heard. The ferrule is now compressed.
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