3Tuning
About Tuning
41 Agilent Triple Quadrupole LC/MS System User Guide
About Tuning
When the MS is used as a detector for a liquid chromatograph, a mass spectrum
is associated with each data point in the chromatogram. To obtain high quality,
accurate mass spectra, the MS must be optimized to:
• Maximize sensitivity
• Maintain acceptable resolution
• Ensure accurate mass assignments.
Tuning is the process of adjusting MS parameters to achieve these goals.
Tuning is primarily concerned with finding the correct settings for the parameters
that control the transmission, filtering, and detection of ions. It is accomplished
by introducing a calibrant into the LC/MS and generating ions. Using these ions,
the tune parameters are then adjusted to achieve sensitivity, resolution, and
mass assignment goals. With a few exceptions, the parameters that control ion
formation are not adjusted. They are set to fixed values known to be good for
generating ions from the calibrant solution.
There are three different types of tunes that can be performed on your
instrument, autotune, checktune, and diagnostic tune. All tunes, except for the
diagnostic tune, can be scheduled to maximize instrument uptime.
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