5 Maintenance
Flushing the Calibrant Delivery System
119 Agilent Triple Quadrupole LC/MS System User Guide
Flushing the Calibrant Delivery System
Schedule
When there is suspected contamination of the CDS, low tune response, or
possible blockage in the CDS, flushing the Calibrant system may be required.
This procedure risks exposure to hazardous, toxic, or flammable solvents and
reagents. Wear appropriate Personal Protective Equipment as described in the
material handling and safety data sheet supplied by the chemical vendor, and
always follow good laboratory practice.
Equipment List
• Clean, powder-free gloves
• Ultrapure LC/MS Grade Acetonitrile
• Ultrapure LC/MS Grade Water
• Tuning solution
Steps
1 Stop the LC/calibrant flow to the MS.
2 Disconnect sample tubing from the top of the nebulizer and place into a clean
beaker or waste bottle.
3 Remove the CDS bottle and discard any old tune solution.
4 Rinse the CDS bottle with LC/MS grade acetonitrile and then LC/MS grade
water.
In the case of a highly contaminated bottle, the bottle can be cleaned with
Alconox and hot water (60 to 70 °C), then rinsed as per step 4.
5 Flush the CDS with 50/50 (v/v) LC/MS grade acetonitrile and LC/MS grade
water:
a Fill the rinsed CDS bottle with approximately 20 to 30 mL of the
acetonitrile/water mixture.
b Install the bottle and turn on the calibrant flow.
c Run the calibrant flush for at least 10 min.
6 Reattach the sample tubing to the top of the nebulizer.
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