3Tuning
Autotune and checktune
Agilent Triple Quadrupole LC/MS System User Guide 42
Autotune and checktune
A checktune is used to determine if the tuning mix ion masses are properly
assigned and if the response or sensitivity of these ions is within expectations. In
other words, a checktune verifies peak width and mass axis to make sure they
are correct before starting a data acquisition. See “Calibrating the LC/TQ
(Checktune)” on page 44.
An autotune only needs to run after preventative maintenance or if there is a
problem with checktune. Everything is automatic since the tuning mix is delivered
by the calibrant delivery system, which is switched on automatically during the
tune. See “Calibrating the LC/TQ (Autotune)” on page 48.
Use Table 2 to select the correct tuning mix for the source installed.
Table 2 Tuning mixes
Source Tuning mix Part Number
ESI ESI-L Low Concentration Tuning Mix G1969-85000
AJS ESI ESI-L Low Concentration Tuning Mix G1969-85000
MMI MMI-L Low Concentration Tuning Mix G1969-85020
APCI APCI-L Low Concentration Tuning Mix G1969-85010