3Tuning
Calibrating the LC/TQ (Autotune)
Agilent Triple Quadrupole LC/MS System User Guide 48
Calibrating the LC/TQ (Autotune)
6475A and Ultivo
An autotune can be run with the following ion sources: ESI, AJS ESI, MMI, and
APCI.
1 In the Method Editor window, click the TQ tab.
2 Click Tune > Autotune in the left pane.
3 Click Request tune control.
This button locks control of the LC/TQ instrument. When tune has control of
the LC/TQ instrument, a single sample run or a worklist cannot be started.
When clicked, tune setpoints are applied. If an action is not selected within the
timeout period, the system will go back to the method setpoints.
4 Select a polarity.
• If only Positive or Negative polarity is selected, subsequence acquisition
methods are only available with that polarity.
• If Both is selected, the system will automatically run in the positive mode
followed by the negative mode.
5 Click Autotune the instrument.
a To stop the tune during operation, click
6 When the tune completes, review the report(s).
7 Click Release tune control to release control of the LC/TQ instrument.
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