Applied Biosystems StepOne - Page 133

DRAFT September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
111
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
f. Confirm that the liquid in each well of the calibration plate is at the bottom of
the well. If not, centrifuge the calibration plate again at a greater rpm and for a
longer time.
IMPORTANT! Do not allow the bottom of the calibration plate to become dirty.
Fluids and other contaminants that adhere to the bottom of the plate can contaminate
the sample block(s) and cause an abnormally high background signal.
2. Load the custom calibration plate into the instrument:
a. Open the instrument drawer.
b. Place the custom calibration plate in the sample block(s) so that the A1 position
is at the back-left corner.
c. Close the instrument drawer carefully.
Run the Custom Spectral Calibration Plate
1. In the main screen of the StepOne
™
software, select Instrument
Instrument Maintenance Manager.
2. In the Instrument Maintenance Manager:
a. In the navigation pane, click Dye.
b. In the Dye screen, select Custom Dye Calibration.
c. Click Start Calibration.
Correct Incorrect
Liquid is at the
bottom of the well.
• Not centrifuged with enough force, or
• Not centrifuged for enough time