Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Glossary.fm
Glossary
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
148
standard curve
method
Method for determining absolute target quantity in samples. With the standard curve
method, the StepOne
™
software measures amplification of the target in samples and in a
standard dilution series. Data from the standard dilution series are used to generate the
standard curve. Using the standard curve, the software interpolates the absolute quantity
of target in the samples. See also standard and standard curve.
standard dilution
series
In standard curve and relative standard curve experiments, a set of standards containing a
range of known quantities. The standard dilution series is prepared by serially diluting
standards. For example, the standard stock is used to prepare the first dilution point, the
first dilution point is used to prepare the second dilution point, and so on. In the StepOne
™
software, the volumes needed to prepare a standard dilution series are calculated by the
number of dilution points, the number of standard replicates, the starting quantity, the
serial factor, and the standard concentration in the stock. See also standard curve.
standard quantity A known quantity in the PCR reaction.
• In standard curve experiments, the quantity of target in the standard. In the
StepOne
™
software, the units for standard quantity can be for mass, copy number,
viral load, or other units for measuring the quantity of target.
• In relative standard curve experiments, a known quantity in the standard. Standard
quantity can refer to the quantity of cDNA or the quantity of standard stock in the
PCR reaction. The units are not relevant for relative standard curve experiments
because they cancel out in the calculations.
starting quantity When defining a standard curve in the StepOne
™
software, corresponds to the highest or
lowest quantity.
step A component of the thermal profile. For each step in the thermal profile, you can set the
ramp rate (ramp increment for melt curve steps), hold temperature, hold time (duration),
and you can turn data collection on or off for the ramp or the hold parts of the step. For
cycling stages, a step is also defined by the AutoDelta status. With StepOnePlus
™
systems, which contain the VeriFlex
™
blocks, each step contains 6 temperatures (1 for
each VeriFlex block).
SYBR
®
Green
reagents
PCR reaction components that consist of two primers designed to amplify the target and
SYBR
®
Green dye to detect double-stranded DNA.

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English