DRAFT
September 25, 2007 1:07 am, 4376782_Glossary.fm
Glossary
149
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
system dye Dye supplied by Applied Biosystems and precalibrated on the StepOne
™
or
StepOnePlus
™
system. Before you use system dyes in your experiments, make sure the
system dye calibration is current in the Instrument Maintenance Manager.
System dyes on the StepOne
™
system:
•FAM
™
dye
•JOE
™
dye
•ROX
™
dye
•SYBR
®
Green dye
•VIC
®
dye
System dyes on the StepOnePlus
™
system:
•FAM
™
dye
•JOE
™
dye
•NED
™
dye
•ROX
™
dye
•SYBR
®
Green dye
•TAMRA
™
dye
•VIC
®
dye
IMPORTANT! Applied Biosystems does not recommend the use of TAMRA
™
dye as
reporter or quencher with the StepOne
™
system. TAMRA dye may be used as a reporter
or quencher with the StepOnePlus
™
system.
TaqMa n
®
reagents PCR reaction components that consist of primers designed to amplify the target and a
TaqMan
®
probe designed to detect amplification of the target.
target The nucleic acid sequence that you want to amplify and detect.
target color In the StepOne
™
software, a color assigned to a target to identify the target in the plate
layout and analysis plots.
Target Library In the StepOne
™
software, a collection of targets to add to experiments. The targets in the
library contain the target name, reporter, quencher, and target color. The target in the
library may also contain comments about the target.
task In the StepOne
™
software, the type of reaction performed in the well for the target or SNP
assay. Available tasks:
• Unknown
•Negative Control
• Standard (standard curve and relative standard curve experiments)
• Positive control (genotyping experiments)
• IPC (presence/absence experiments)
• Blocked IPC (presence/absence experiments)
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