Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Glossary.fm
Glossary
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
150
temperature plot In the StepOne
™
software, a display of temperatures for the sample, instrument cover, and
instrument block during the StepOne
™
or StepOnePlus
™
instrument run.
template In the Design Wizard of the StepOne
™
software (and in QuickStart for quantitation
experiments), the type of nucleic acid to add to the PCR reaction. The recommended
template varies according to experiment type:
• Quantitation experiments (standard curve, relative standard curve, and comparative
C
T
) – cDNA (complementary cDNA), RNA, or gDNA (genomic DNA)
For quantitation experiments, the template type selection affects the run method,
reaction setup, and materials list.
• Genotyping experiments – Wet DNA (gDNA or cDNA) or dry DNA (gDNA or
cDNA)
For genotyping experiments, the template type selection affects the reaction setup.
• Presence/absence experiments - DNA
For presence/absence experiments, Applied Biosystems recommends adding DNA
templates to the PCR reactions.
thermal profile Part of the run method that specifies the temperature, time, ramp, and data collection
points for all steps and stages of the StepOne
™
or StepOnePlus
™
instrument run.
threshold 1. In amplification plots, the level of fluorescence above the baseline and within the
exponential growth region The threshold can be determined automatically (see
automatic C
T
) or can be set manually (see manual C
T
).
2. In presence/absence experiments, the level of fluorescence above which the
StepOne
™
software assigns a presence call.
threshold cycle (C
T
) The PCR cycle number at which the fluorescence meets the threshold in the amplification
plot.
Tm See melting temperature (Tm).
touchscreen Instrument display that you touch to control the StepOne
™
or StepOnePlus
™
instrument.
unknown In the StepOne
™
software, the task for the target or SNP assay in wells that contain the
sample you are testing:
• In quantitation experiments, the task for the target in wells that contain a sample
with unknown target quantities.
• In genotyping experiments, the task for the SNP assay in wells that contain a sample
with an unknown genotype.
• In presence/absence experiments, the task for the target in wells that contain a
sample in which the presence of the target is not known.
unknown-IPC wells In presence/absence experiments, wells that contain a sample and internal positive control
(IPC).

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English