EasyManuals Logo

Applied Biosystems StepOne User Manual

Applied Biosystems StepOne
184 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
To Previous Page IconTo Previous Page
To Previous Page IconTo Previous Page
Page #173 background imageLoading...
Page #173 background image
DRAFT
September 25, 2007 1:07 am, 4376782_Glossary.fm
Glossary
151
Applied Biosystems StepOne
and StepOnePlus
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
VeriFlex
Technology
The StepOnePlus
instrument contains six independently thermally regulated VeriFlex
blocks, creating up to six different zones for the 96 sample wells. After you enable the
VeriFlex blocks in the StepOne
software, you can set a different temperature for one or
more of the VeriFlex blocks.
y-intercept In the standard curve, the value of y where the regression line crosses the y-axis. The y-
intercept indicates the expected threshold cycle (C
T
) for a sample with quantity equal to 1.
zone One of up to six sample temperatures among the 96 wells formed by independently
thermally regulated VeriFlex
blocks during the StepOnePlus
instrument run. You can
set a different temperature for one or more of the VeriFlex blocks, or you can set the same
temperature for each of the VeriFlex blocks.
Note: For melt curve steps, you need to set the same temperature for each of the
VeriFlex blocks.
zone boundary The edge of a zone for samples formed by the six independently thermally regulated
Ver i Fl ex
blocks. In the StepOne
software, the zone boundaries are displayed in the
plate layout as thick red lines.

Table of Contents

Questions and Answers:

Question and Answer IconNeed help?

Do you have a question about the Applied Biosystems StepOne and is the answer not in the manual?

Applied Biosystems StepOne Specifications

General IconGeneral
BrandApplied Biosystems
ModelStepOne
CategoryLaboratory Equipment
LanguageEnglish

Related product manuals