Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Glossary.fm
Glossary
151
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
VeriFlex
™
Technology
The StepOnePlus
™
instrument contains six independently thermally regulated VeriFlex
™
blocks, creating up to six different zones for the 96 sample wells. After you enable the
VeriFlex blocks in the StepOne
™
software, you can set a different temperature for one or
more of the VeriFlex blocks.
y-intercept In the standard curve, the value of y where the regression line crosses the y-axis. The y-
intercept indicates the expected threshold cycle (C
T
) for a sample with quantity equal to 1.
zone One of up to six sample temperatures among the 96 wells formed by independently
thermally regulated VeriFlex
™
blocks during the StepOnePlus
™
instrument run. You can
set a different temperature for one or more of the VeriFlex blocks, or you can set the same
temperature for each of the VeriFlex blocks.
Note: For melt curve steps, you need to set the same temperature for each of the
VeriFlex blocks.
zone boundary The edge of a zone for samples formed by the six independently thermally regulated
Ver i Fl ex
™
blocks. In the StepOne
™
software, the zone boundaries are displayed in the
plate layout as thick red lines.

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English