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5.6 Decontamination
Decontaminate after spills, splashes, or obvious contamination, and before transporting the
equipment out of the laboratory.
WARNING
CONSIDER ALL ORGANISMS AS POTENTIALLY INFECTIOUS AND HANDLE
ACCORDING TO STANDARD MICROBIOLOGICAL PRACTICES, SPECIAL
PRACTICES, AND SAFETY EQUIPMENT RECOMMENDED FOR BIOSAFETY
LEVEL 2 (BSL-2) CONTAINMENT.*
BSL-2 PRACTICES INCLUDE, BUT ARE NOT LIMITED TO THE FOLLOWING:
l
WEAR GLOVES AND LAB COAT WHEN HANDLING POTENTIALLY
INFECTIOUS MATERIAL OR CONTAMINATED EQUIPMENT. WEAR ANY
OTHER PERSONAL PROTECTIVE EQUIPMENT ACCORDING TO LOCAL
POLICY.
l
DECONTAMINATE WORK SURFACES AFTER COMPLETION OF WORK AND
AFTER ANY SPILL OR SPLASH OF POTENTIALLY INFECTIOUS MATERIAL
WITH APPROPRIATE DISINFECTANT.
l
DECONTAMINATE ALL CULTURES, STOCKS, AND OTHER POTENTIALLY
INFECTIOUS MATERIALS BEFORE DISPOSAL USING AN EFFECTIVE
METHOD.
l
PERFORM ALL PROCEDURES TO MINIMIZE THE CREATION OF SPLASHES
AND/OR AEROSOLS.
l
LABORATORY EQUIPMENT SHOULD BE ROUTINELY DECONTAMINATED, AS
WELL AS, AFTER SPILLS, SPLASHES, OR OTHER POTENTIAL
CONTAMINATION. SPILLS INVOLVING INFECTIOUS MATERIALS MUST BE
CONTAINED, DECONTAMINATED, AND CLEANED UP BY STAFF PROPERLY
TRAINED AND EQUIPPED TO WORK WITH INFECTIOUS MATERIAL.
l
EQUIPMENT MUST BE DECONTAMINATED BEFORE REPAIR, MAINTENANCE,
OR REMOVAL FROM THE LABORATORY.
l
DISPOSE OF ALL USED REAGENTS AND ANY OTHER CONTAMINATED
DISPOSABLE MATERIALS FOLLOWING PROCEDURES FOR INFECTIOUS OR
POTENTIALLY INFECTIOUS WASTE. IT IS THE RESPONSIBILITY OF EACH
LABORATORY TO HANDLE SOLID AND LIQUID WASTE ACCORDING TO
THEIR NATURE AND DEGREE OF HAZARDOUSNESS AND TO TREAT AND
DISPOSE OF THEM (OR HAVE THEM TREATED AND DISPOSED OF) IN
ACCORDANCE WITH ANY APPLICABLE REGULATIONS.
*
Biosafety in Microbiological and Biomedical Laboratories, 6th Edition. 2020. U.S. Department of Health
and Human Service, Centers for Disease Control and Prevention and National Institutes of Health. Refer
to Biosafety in Microbiological and Biomedical Laboratories on cdc.gov.
BD Kiestra™ ReadA User's Manual
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