PN 177196BB
7-14
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS - MEASUREMENT PRINCIPLES
9. The DiffPlot is developed with optical transmission (absorbance) on the X-axis and
volume on the Y-axis
a. DiffPlot used to determine four of five leukocyte (white blood cell) populations:
lymphocytes (
LY), monocytes (MO), neutrophils (NE), and eosinophils (EO).
b. Changes in the morphology of a population are expressed on the DiffPlot by a
shifting of the corresponding population.
1) Volume and absorbance thresholds are used to detect shifting populations.
2) Most of the population partition thresholds are fixed and give the limits of the
morphological normality of leukocytes
Typical Absorbance Signals
Combining Volume and Absorbance Data to Create a DiffPlot