PN 177196BB
7-3
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS - AN OVERVIEW OF THE DILUTIONS AND MEASUREMENTS
7
7.2 DATA ANALYSIS - AN OVERVIEW OF THE DILUTIONS AND MEASUREMENTS
A Objectives
When you have completed this topic, you will be able to:
B State the parameters derived from the dilutions made in each bath.
B Briefly explain the sequential dilution system (SDS)
B References
1. Bulletin 9151: A
c
V Differential Technology and Case Studies.
2. In the Online Help System or the Instructions for Use manual, refer to:
r
Heading 2.5 SAMPLE ANALYSIS OVERVIEW.
r Heading 2.6 SAMPLE ANALYSIS.
C Special Instructions
The information in this topic is versatile and could be used as a lead in or a review for several
different topics, so just follow any special instructions for the topic you decide to use it with.
D Topic Notes and Tasks
Preparing the Dilutions for Sample Analysis
1. During aspiration, whole blood is pulled into the sampling probe.
a. 30 µL of whole-blood is aspirated for a CBC panel.
b. 53 µL of whole-blood is aspirated for a CBC/DIFF panel.
c. The volume of sample aspirated from the specimen tube into the sampling probe is
sufficient to make all the dilutions needed to develop the parameter results for the
selected test panel.
2. The sample is partitioned as it is distributed to the baths in the baths assembly.
a. Use the illustrations on the next page as needed to explain the partitioning of the
sample and the sequential dilution system (SDS).
b. This information is covered under
Heading 2.8, SAMPLE ANALYSIS OVERVIEW, in the
Online Help System or the Instructions for Use manual.
3. Dilutions are made in three of the baths for a CBC panel analysis:
a. DIL1/HGB bath
b. RBC bath
c. WBC/BASO bath
4. Dilutions are made in four of the baths for a CBC/DIFF panel analysis:
a. DIL1/HGB bath
b. DIFF bath
c. RBC bath
d. WBC/BASO bath
5. Steps required to make these dilutions are detailed under Heading 8.2, RECOGNIZING
NORMAL INSTRUMENT OPERATION - CYCLE DESCRIPTION, in this Training Guide.
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