PN 177196BB
7-28
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS REVIEW - PLATELET PARAMETERS
2. Rinse flow is a steady stream of Diluent reagent that flows behind the RBC aperture
during sensing periods.
a. Without rinse flow (Figure A), there is a characteristic swirling of the dilution at the
outlet of the aperture so that red (or white) cells caught up in these eddies behind
the aperture may reenter the sensing zone and produce small pulses that could be
counted as platelets.
b. With rinse flow (Figure B), the steady stream of Diluent prevents the formation of
these eddies.
3. To obtain a Plt count result, the Analyzer compares and votes on the data from the two
5-second count periods.
a. If two counts agree, Plt count reported.
Plt count = Number of cells counted per volume unit x calibration factor
Plt count displayed and printed as: Plt = N x 10
3
cells /µL
b. If two counts differ more than the predefined limit, Plt result is flagged with a V.
PLT Histogram
1. Platelets are categorized according to size by a 256-channel pulse-height analyzer.
a. Pulse-height analyzer uses a number of thresholds to sort the particles into several
size (volume) categories and to develop a size distribution curve of the particles
between 2 fL and 30 fL.
b. Platelet histogram reflects the native size of the platelets and any other particle in
the platelet size range.
ensing
zone
Swirling
effect
No
rinse
flow
AB
Cell
Rinse
flow
Cell
Sensing
zone
To
waste
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