Beckman Coulter COULTER ACT 5diff - 7.4 DATA ANALYSIS REVIEW - WBC PARAMETERS

PN 177196BB
7-15
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS REVIEW - WBC PARAMETERS
7
7.4 DATA ANALYSIS REVIEW - WBC PARAMETERS
A Objectives
When you have completed this topic, you will be able to:
B List the individual parameters that comprise the WBC Profile.
B Identify which parameters in the WBC Profile are directly measured, derived from the
WBC/BASO histogram, derived from the DiffPlot, or computed.
B Identify the flags that occur when the WBC count results do not agree.
B Demonstrate how to locate details concerning flags, interpretive messages, or analytical
alarms in the Online Help System or the Instructions for Use manual.
B References
1. Bulletin 9151: A
c
V Differential Technology and Case Studies.
2. In the Online Help System or the Instructions for Use manual, refer to:
r Heading 2.7 PARAMETER DEVELOPMENT.
r Heading 9.7 REVIEWING RESULTS.
r Heading 9.8 FLAGS AND MESSAGES GENERATED BY THE INSTRUMENT.
C Special Instructions
This topic is best covered in a classroom setting. Having a computer available to show where
this information is located in the Instructions for Use Manual is desirable.
D Topic Notes and Tasks
WBC Counts
1. The reference WBC count is determined from the 1:200 dilution made in the
WBC/BASO bath.
2. The WBC Lyse reagent in the dilution:
a. Lyses the RBCs.
b. Specifically differentiates between basophils and other WBCs by volume.
3. To obtain a WBC count, the Analyzer compares and votes on data from two 6-second
count periods.
a. If the two counts agree, the reference WBC counts reported.
WBC count = Number of cells per volume x calibration factor
WBC count displayed and printed as: WBC = N x 10
3
cells /µL
b. If the two counts differ more than predefined limit, WBC result is flagged with a
voteout V-flag and the DIFF absolute numbers are also flagged with a V.