PN 177196BB
7-16
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS REVIEW - WBC PARAMETERS
4. When the CBC/DIFF test panel is selected, a second WBC count is analyzed on the DIFF
dilution in the flow cell during the acquisition of the DiffPlot.
a. This WBC count is sometimes referred to as the secondary WBC count.
b. This WBC count is compared to the WBC count from the WBC BASO bath and if
the difference exceeds a preset limit, the WBC results are flagged.
1) If the WBC count from the flow cell exceeds the WBC count from the
WBC/BASO bath by more than a predefined amount, DIFF+ is displayed.
2) If the WBC count from the flow cell is less than the WBC count from the
WBC/BASO bath by more than a predefined amount, DIFF- is displayed
3) When a DIFF- or a DIFF+ flag occurs, the WBC count and all DIFF#
parameters are flagged with an * (asterisk).
BASO Count
1. The basophils are determined simultaneously with the WBC count from the final 1:200
dilution in the WBC/BASO bath.
a. Raw data for developing the WBC/BASO histogram is collected through the two
consecutive time periods of 6-seconds each (for a total of 12 seconds).
b. Differentiation between the basophils and the other WBC is accomplished by means
of the WBC Lyse-specific lytic action.
2. All the WBCs (including the basophils) are included on the WBC/BASO histogram.
a. One hundred percent (100%) of the leukocytes is represented by the total number
of nucleated particles plus the basophils within area between thresholds labeled
b and d.
b. Basophils are located in the area between thresholds labeled
c and d.
c. Basophil percentage is calculated from number of particles existing in the area
between thresholds labeled
c and d
d. BASO count = Number of cells per volume x calibration factor in a percentage
relative to the number of counted cells (basophils plus other WBC nuclei)
basophilsWBC
bc d
BASO count
BASO%
WBC%
----------------------
WBC count×=
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