PN 177196BB
7-21
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS REVIEW - RBC PARAMETERS
7
7.5 DATA ANALYSIS REVIEW - RBC PARAMETERS
A Objectives
When you have completed this topic, you will be able to:
B List the individual parameters that comprise the RBC Profile.
B Identify which parameters in the RBC Profile are directly measured, derived from the
RBC histogram, or computed.
B Compute MCV, MCH, and MCHC results.
B Explain the process of building an RBC histogram.
B Describe a typical RBC histogram.
B Identify the flags that occur when the RBC count results do not agree.
B Demonstrate how to locate details concerning flags, interpretive messages, or analytical
alarms in the Online Help System or the Instructions for Use manual.
B References
1. Bulletin 9151: A
c
V Differential Technology and Case Studies.
2. In the Online Help System or the Instructions for Use manual, refer to:
r Heading 2.7 PARAMETER DEVELOPMENT.
r Heading 9.7 REVIEWING RESULTS.
r Heading 9.8 FLAGS AND MESSAGES GENERATED BY THE INSTRUMENT.
C Special Instructions
This topic is best covered in a classroom setting. Having a computer available to show where
this information is located in the Instructions for Use Manual is desirable.
D Topic Notes and Tasks
RBC Count
1. The RBC count is obtained from the RBC/PLT dilution in the RBC bath.
a. Final 1:10,000 dilution in RBC bath that is often referred to as RBC/PLT dilution.
b. RBC dilution contains red blood cells, white blood cells, and platelets.
1) Thresholds are used to separate platelet pulses, which are much smaller, from
the red and white blood cell pulses.
2) Since white blood cells fall in red blood cell size range, they are counted and
sized as RBCs.
2. To obtain an RBC count result, the Analyzer compares and votes on data collected for
two 6-second count periods.
a. If the two counts agree, the RBC count is reported.
RBC count = Number of cells counted per volume unit x calibration factor
RBC count displayed and printed as: RBC = N x 10
6
cells /µL
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