PN 177196BB
7-25
DATA MANAGEMENT AND REVIEW
DATA ANALYSIS REVIEW - RBC PARAMETERS
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3. The system calculates the Hgb using both the blank and sample readings:
Hgb result in g/dL represents: absorbance value obtained x calibration factor
Hgb displayed and printed as: Hgb = N g/dL
4. If the Hgb blank or Hgb read do not meet certain predefined limits, the following flags
are generated:
a. If the Hgb blank value is less than 2.5 Vdc, a reject R-flag occurs on the Hgb value.
b. If the difference between the new Hgb blank reference value and the original Hgb
blank reference value is greater than 3%, review R-flag is generated
c. If three consecutive review R-flags occur on the Hgb blank reference value, (
. . . .)
code replaces the Hgb, MCH, and MCHC results.
d. If the difference between the three reading taken on the Hgb sample exceeds the
predefined limits, a voteout V-flag is generated.
5. If the Hgb to Hct ratio is <0.8 or >1.2, the RBC, Hgb, MCV, Hct, MCH, MCHC, Plt, MPV,
Pct, and PDW are flagged with *, indicating a possible error in the analytical process.
The calculation for the Hgb to Hct ratio is:
MCV Calculation
1. MCV (Mean Cell Volume) is calculated using the Hct and the RBC count.
2. Displayed and printed in femtoliters (fL)
Note: 1fL = 1µL
3
3. Calculation for MCV is:
MCH Calculation
1. MCH (Mean Cell Hemoglobin) is calculated from the Hgb value and the RBC count and
describes the average weight of hemoglobin in a red blood cell.
2. Displayed and printed in picograms (pg)
3. Calculation for MCH is:
MCHC Calculation
1. MCHC (Mean Cell Hemoglobin Concentration) is calculated using the Hgb and Hct
values and describes the average concentration of hemoglobin in the red blood cells.
2. Displayed and printed in grams per deciliter
3. Calculation for MCHC is:
Hgb g/dL 3×
Hct %
--------------------------------
Hct
RBC
------------
10 × MCV (fL)=
Hgb
RBC
------------
10 × MCH (pg)=
Hgb
Hct
----------
100 × MCHC (g/dL)=
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