PN 177196BB
8-15
SYSTEM DIAGNOSTICS AND MAINTENANCE
RECOGNIZING NORMAL INSTRUMENT OPERATION - CYCLE DESCRIPTION
8
Dilution Summary
Rinsing the Baths
1. After analysis of the dilution in the WBC/BASO
bath, the horizontal traverse assembly moves to
the WBC/BASO bath and the WBC/BASO bath is
drained.
2. The sampling probe is rinsed with Diluent and
Rinse reagent (cleaning agent) is delivered into
the WBC/BASO bath.
Note: This mixture of Diluent and Rinse reagent
is pulled through the aperture in the
WBC/BASO bath, cleaning that aperture and
providing a restricted flow of reagent for the
sweep flow behind the RBC/Plt aperture.
3. After analysis of the dilutions in the DIL 1/HGB,
DIFF, and RBC baths, the baths are drained and
clean Diluent is added.
4. The hemolgobin blank is read on the clean
Diluent in the DIL 1/HGB bath.
Preparing for the Next Sample
1. The horizontal traverse assembly returns to the
sampling position.
2. The sampling syringe moves down creating the
air gap for the next cycle.
DIL 1
HGB
DIFF RBC
BASO
WBC
RINSE
Bath Dilution Components Final Dilution Ratio Final Dilution Function
DIL1/HGB 1. 10 µL whole-blood sample + 1.7 mL Diluent
2. 42.5 µL of the first dilution is removed
0.40 mL of Diluent added while probe is still in bath
3. 0.4 mL Hgb Lyse reagent added
1:250 Hgb measurement
DIFF 1. 25 µL whole-blood sample + 1.0 mL Fix reagent
2. 12 second incubation
3. 1.0 mL of Diluent added
1:80 DiffPlot development
and secondary WBC
count
RBC 1. 42.5 µL of the first 1:170 dilution
2. 2.5 mL of Diluent added
1:10,000 RBC count and RBC
and PLT histograms
development
WBC/BASO 10 µL whole-blood sample + 2.0 mL WBC Lyse reagent 1:200 WBC count and
WBC/BASO histogram
development
To Next Page
Loading...
Need help?
General
