48 | Chapter 3: Operation
BioTek Instruments, Inc.
Single or Dual Wavelength
W A V E L E N GTH: DUAL
S I N G L E DUAL
1. Select SINGLE or DUAL wavelength.
2. Press ENTER to continue.
The WAVELENGTH selection screen allows you to select SINGLE or DUAL
wavelength for the assay.
If SINGLE wavelength is chosen, the reader measures the optical density of each
well with a single filter.
If DUAL wavelength is chosen, each well is read twice, each time with a different
filter. The microplate is not removed from the reading chamber between the two
measurements. The final reported optical density is the difference between the two
readings (the delta OD). Dual-wavelength readings can significantly reduce optical
interference caused by scratched or fingerprinted microplates, since the scratches
or fingerprints reduce the amount of light on both wavelengths.
The currently selected wavelength appears on the top line of the display, and the
available options appear on the bottom.
MEAS Selection
The MEAS selection screen allows you to select the filter(s) for the assay.
M E A S : 4 5 0 REF:630
4 0 5 4 5 0 4 9 0 6 3 0
1. Select the wavelength.
2. Use the right arrow key to move the cursor to REF and then select the
Reference Filter.
3. Press ENTER to move to the next screen.
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