Dirui CS-T240 - Two Points Analysis Method; 2-Point Assay Reaction Curve

77
Example 2、UA（uric acid）－Uric acid liquid reagent kit
wavelength
Main 520nm
（
500,550
optional
）
Absorbance
range
0
～
2A
Test mode
Endpoint analysis method
Optical path
10mm
Reagent
1
：
200uL
；
2
：
50uL
sample
4uL
Single
reagent
4 parts of reagent 1 part of
reagent 2
Mixing storage
2
～
8
℃
5 days stablity
temperature
37
℃（
30
℃, 2 5 ℃ ）
incubation
5min
（
6min , 8min
）
reaction
5min
adjust “0”
520nm
，
blank pipe
sensitivity
0.42mA equals to1umol/L
Linearity range
1.5mmol/L
（
25mg/dL
）
calibration
0.72mmol/L
，
0.302A
Unit
conversion
1mmol/L
＝
16.8mg/dL
Reference
value
child
：
0.12
～
0.33mmol/L
（
2.0
～
5.5mg/dL
）；
Male：0.21～0.43mmol/L（3.5～7.2mg/dL）；
female：0.15～0.36mmol/L（2.5～6.0mg/dL）；
urine
：
14.9
～
44.6mmol/L
（
250
～
750mg/dL
）；
8.4 Two Points analysis method
Two points analysis method (fixed time assay) is also named as first class
dynamics analysis method, which means during a certain period time of reaction,
reaction speed is in direct proportion to the simple power of substrate
concentration in specified time, namely v=k[S]. Due to the reduction of
substrate, the whole reaction speed is decreasing gradually, which shows the
increase (decrease) of absorbance. Because it takes a very long time to reach
balance, it can be monitored at any time theoretically, but due to the
complexity of serum ingredient, it must take a certain period of time to enter
into stable reaction phase.
Figure 8-2 2-point assay reaction curve
conversion
Reference
value
Adult
；
5.1
～
19umol/L
（
0.3
～
1.1mg/dL
）
Newly born：20～200umol/L（1.2～12mg/dL）
Cell blank
Reaction limit level
Absorbance
Absorbance
Time
Time