Dirui CS-T240 - Chapter 8 Analysis Method; 8.1 Analysis Principle

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Select and execute “cuvette blank test” after click “System maintenance”, and
observe execution result and reaction status.
Chapter 8 Analysis Method
CS-T240 adopts three analysis methods as follows:
End point analysis method
Two-points analysis method
Rate analysis method
8.1 Analysis principle
The analysis principle of auto-chemistry analyzer is based on the Beer-Lambert law that material
absorpts light selectively.
The main principle is: When monochromatic light with specific wavelength passes through the cuvette
with sample, the monochromatic light absorbency and sample liquid concentration vary in positivw
proportion as the distance of the light possing through the liquid.
c b
I
I
lg1/TlgA
0
ε === ）（）（
t
A －Absorbency of the light when passes through liquid
T －Transmitted intensity and incident intensity ratio: transmittance I
t
/I
0
；
I
0
－ Incident intensity
I
t
－ Transmitted intensity
ε － Molar absorption coefficient of solution（ml×mmol－1×cm－1）；
c － Mol concentration of the solution（mmol/ml）；
b － Solution layer thickness（cm）；
Solution layer thickness (b): Optical path, which is fixed by instrument. Molar absorption
coefficient (ε) is the correlation coefficient of the wavelength, solution and solution temperature.
Linear relationship is displayed between solution thickness and absorbency when in stable temperature
and single wavelength（ε value is given on the reagent bottle by factory）