Two points end point analysis two points rate analysis
8.5 Rate Analysis
Rate analysis, also known as zero-class dynamics analysis, refers to the
reaction rate is directly proportional to the zero power of substrate
concentration, which has nothing to do with the substrate concentration. Hence,
the reactants can generate a certain product at constant speed throughout the
reaction process, resulting in even decrease or increase of absorbance of
measured solution at a wavelength, and the decrease or increase speed (△ A
/ min) is directly proportional to the activity or concentration of the tested
substance (catalytic material). Dynamics assay is also called as the
continuous monitoring assay, mainly used for the measurement of enzyme
activity.
In fact, because substrate concentration is not high enough, with the reaction
proceeds, the reaction is no longer zero class when substrate is consumed to
a certain extent, Therefore, zero-class dynamics analysisn is targeted at a
certain period of time; Because reaction time to reach balance is very long,
it can be monitored at any time theoretically, but because of the complexity
of serum ingredient and much reaction, therefore, it takes a certain period
of time to enter in stable reaction phase. So all reagent manufactures have
strict requirements to thesse two time periods
Dynamics analysis is based on the changes between specified photometric points
to obtain the absorbance concentration or activity value.
Metering point in accordance with the input form, dynamics method can be
divided into single-band and dual-band dynamics analysis.
Figure 8-3 Rate assay reaction curve
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