Leica DMi8 User Manual

CONTENTS
6. Troubleshooting ......................................................... 86
7. Care of the Microscope ............................................ 89
7.1 Dust Cover ................................................................... 89
7.2 Cleaning ....................................................................... 89
7.3 Handling acids and bases ........................................ 90
8. Essential Consumable and Spare Parts ............... 91
9. Appendix .................................................................... 92
9.1 Assigning the variable function keys ..................... 92
9.2 Abbreviation glossary ............................................... 94
9.3 Overview of Touch Screen pictograms .................. 95
9.4 Additional information ............................................... 96
10. Index ............................................................................ 97
11. EC Declaration of Conformity .................................. 99
5. Operation .................................................................... 38
5.1 Switching on the microscope .................................. 39
5.2 Illumination ................................................................. 41
5.2.1 Transmitted Light........................................................ 41
5.2.2 Fluorescence .............................................................. 42
5.2.3 Infinity port .................................................................. 44
5.2.4 Shutter ......................................................................... 45
5.3 Objectives ................................................................... 46
5.4 Tube .............................................................................. 49
5.5 Eyepieces .................................................................... 50
5.6 Stages and object displacement ............................. 51
5.6.1 Object displacement ................................................. 51
5.6.2 Adjusting the step sizes on motorized stages ....... 52
5.7 Focusing ...................................................................... 54
5.7.1 Focusing the image ................................................... 54
5.7.2 Focus thresholds ........................................................ 55
5.7.3 Adjusting the step sizes ............................................ 56
5.7.4 AFC (Adaptive Focus Control) .................................. 57
5.8 Selecting the ports .................................................... 58
5.9 Magnification Changer ............................................. 59
5.10 Aperture diaphragm and field diaphragm ............. 60
5.11 Filter ............................................................................. 62
5.11.1 Transmitted Light filter .............................................. 62
5.11.2 Fluorescence Cubes .................................................. 63
5.11.3 Leica RFID Module......................................................... 65
5.11.3.1 Product Description ................................................. 65
5.11.3.2 Specifications LID Module 1.0 ...............................65
5.11.3.3 Specifications RFID Tag D6.7 special ..................... 65
5.11.3.4 Ports ............................................................................. 65
5.11.3.5 FCC / IC Compliance .................................................. 65
5.12 The memory function on the Touch Screen .......... 66
5.13 Transmitted Light contrast method ......................... 66
5.13.1 Brightfield (TL) ............................................................ 67
5.13.2 Phase Contrast (TL) ................................................... 69
5.13.3 Darkfield (TL) .............................................................. 71
5.13.4 Polarization (TL) ......................................................... 72
5.13.5 Differential Interference Contrast – DIC (TL) ....... 74
5.13.6 Integrated Phase Contrast – IPH (TL) .................... 76
5.13.7 Integrated Modulation Contrast – IMC (TL) .......... 78
5.14 Fluorescence .............................................................. 82
5.15 Combination method ............................................... 85
3

Leica DMi8 Specifications

General

Type	Inverted Microscope
Software	Leica Application Suite X (LAS X)
Dimensions	Varies depending on configuration
Weight	Varies depending on configuration
Imaging Modes	Brightfield, Phase Contrast, DIC, Fluorescence
Objective Compatibility	Leica Objectives (various threads depending on nosepiece)
Stage	Motorized XY stage
Camera Compatibility	Leica cameras and 3rd party cameras via C-mount or direct camera ports
Illumination	LED illumination
Focusing	Motorized Z-drive
Nosepiece	Motorized 6-position nosepiece
Automation	Fully automated
Optical System	Infinity Optical System
Magnification Range	Varies depending on objective used
Observation Tubes	Various ergonomic observation tubes available (binocular, trinocular)
Filter Slots	Multiple filter cube slots
Camera Port	C-mount
Software Compatibility	Compatible with Leica LAS X software