Leica DMi8 - IMC (TL)

5. OPERATION - TRANSMIT TED LIGHT CONTR AST ME THOD
5.13.6 Integrated Phase Contrast – IPH (TL)
Note:
The Integrated Phase Contrast requires a condenser on the
tiltable Transmitted Light arm.
Leica DMi8 with manual Transmitted Light method
t*GOFDFTTBSZTFUUIF5-#SJHIUmFMEQPTJUJPOPSBOFNQUZ
position on the fluo turret, or select the "DAPI-TL" filter
cube.
t4FMFDUB#SJHIUmFMEPCKFDUJWFXJUIFZFQPJOU#PS$
t4FMFDUUIFDPSSFTQPOEJOHlight ring on the condenser (see
table).
t$PNQMFUFMZPQFOUIFBQFSUVSFPOUIFDPOEFOTFS
t4XJOHBMMSFNBJOJOHPQUJDBMDPNQPOFOUTTVDIBTUIFBOB-
lyzer, polarizer or IC prisms, from the beam path.
t*GOFDFTTBSZSFNPWFUIFDPWFSPGUIFIPMEFSBOEJOTFSU
the Phase Contrast module evenly from the front on the
left side of the microscope.
t4MJEFUIF1IBTF$POUSBTUNPEVMFJOUPUIFDPSSFDUFZFQPJOU
B or C.
t*OTFSUB1IBTF$POUSBTUTQFDJNFO
t'PDVTUIFJNBHFVTJOHUIFGPDVTEJBMT
t$POmHVSFUIFCSJHIUOFTTPOUIFJOUFOTJUZSPUBSZLOPC
IP0 for 5x e.g. NPlan 5x Objective with eyepoint B
IP1 for 10x e.g. NPlan 10 x Objectives with eyepoint B and
for 20x e.g. NPlan L 20 x Objectives with eyepoint C
IP2 for 40x e.g. HCX PL FL L 40 x Objectives with eyepoint C
IP3 for 63x e.g. PL FL 63x/0.70 Objectives with eyepoint C
Phase Contrast module for tiltable Transmitted Light illumi-
nation carrier
1 Centering screws for light ring 20x - 63x
2 Position for objectives 20x - 63x
3 Brightfield position
4 Position for objectives 5x - 10x
5 Phase ring 20x - 63x
6 Phase ring 5x - 10x
7 Fastening screw for light ring 20x - 63x
Receptacle for Phase Contrast module
12345
6
7
76