Leica DMi8 - Adaptive Focus Control (AFC)

5. OPERATION - FOCUSING
5.7.4 AFC (Adaptive Focus Control)
Leica DMi8 with motorized focus/closed loop
The AFC can be used to actively hold a defined focus posi-
tion and automatically readjust this position regularly. This
is useful if, for example, a temperature change is caused by
opening the climate chamber during living cell experiments
at 37°C.
The AFC is activated, then the specimen on the stand is
focused using the handwheel and the stopping position is
saved.
t"'$NPOJUPSJOHDBOCFQFSGPSNFEVTJOHWBSJBCMFGVODUJPO
keys, LAS X software or via the Touch Screen.
Setting the AFC via the Touch Screen
t0OUIF5PVDI4DSFFOVTFUIF
key for switching to
stage and focus controls.
t4FMFDUUIFAdaptive Focus Control menu using the
key.
t"DUJWBUFUIF"'$VTJOHUIFOn/Off key. The knob behind
AFC turns green.
t'PDVTUIFTQFDJNFOBOETBWFUIFQPTJUJPOVTJOHUIFHold
key.
t*GUIFQPTJUJPOJTIFMEUIFLOPCCFIJOEPosition will turn
green.
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