Leica DMi8 - Darkfield (TL)

5. OPERATION - TRANSMIT TED LIGHT CONTR AST ME THOD
5.13.2 Phase Contrast (TL)
(Integrated Phase Contrast, see 5.13.6)
Leica DMi8 with manual Transmitted Light method
t*GOFDFTTBSZTFUUIF5-#SJHIUmFMEQPTJUJPOPSBOFNQUZ
position on the fluo turret, or select the "DAPI-TL" filter
cube.
t4FMFDUB1IBTF$POUSBTUPCKFDUJWF0CKFDUJWFTUIBUBSF
suitable for Phase Contrast are engraved with PH.
For a tiltable Transmitted Light arm:
t4FMFDUUIFDPSSFTQPOEJOHlight ring on the condenser.
For a fixed Transmitted Light arm:
t$POmHVSFUIFDPOEFOTFSIFJHIUBOESFNPWFUIF*.$
slit diaphragm slider, if necessary.
t(VJEFUIF1IBTF$POUSBTU light ring slide with the
marking "TOP LEFT" on the top left laterally into the re-
ceptacle until it clicks into place.
(Caution: there are various slide bars for the condens-
ers!)
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to the magnification (5, 10/20 or 40).
Note:
The Phase Contrast light ring slide is encoded. As soon as
the slider is taken out of the Brightfield position (available
opening) and engaged in a Phase Contrast position (any
light ring), the brightness increases. Conversely, switch-
ing from Phase Contrast to Brightfield decreases the
brightness.
1 Slider for light rings (S80/0.30)
2 Slider for light rings (S40/0.45)
3 Click stop
4 Light rings
5 Brightfield position
6 "TOP LEFT" marking
3
1 2
4
5
4
4
6 6
Phase Contrast for fixed Transmitted Light arm
Light ring slide for fixed Transmitted Light arm
Condenser setting for tiltable Transmitted Light arm - con-
figuring light ring, e.g. PH1
Light ring slide
69