Lonza 4D-Nucleofector - Page 29

4D-Nucleofector
®
Manual | 29
1. Catridge inseted in slot
2. Shot venting tube with filter attached to the male Luer
connector on the front plate
3. Tubes inseted in pinch valves (press black round
surface of the valves while pulling tubes fully into the
valves)
4. T connector inseted into T connector holder. Two
larger diameter tubes completely inseted into liquid
sensors
5. Upper tube (for cell suspension!) inseted into the
upper pump. Flap closed.
6. Optional - when feeding substrate separately - lower
tube inseted into lower pump. Flap closed.
7. Holder for cell suspension reservoir placed on a
magnetic stirring platform (stirring speed to approx.
300 rpm)
8. All reservoir-tube connections established to
a. Input reservoir for cell suspension
b. Input reservoir for substrate (optional)
c. Output reservoir
Figure 2.38: Setup Checklist for LV Nucleocuvette
®
Catridge. The picture
reflects the GMP-grade TheraPEAK
®
Consumables, research-grade versions
may slightly differ but the mounting procedure stays the same.
2
8c8b
4
6
5
7
8a
3
1
2
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