REF : M18-SM-001 Rev 02
2. FLUIDICS
MYTHIC 18 Copyright© Orphée SA. All Rights Reserved. Page 9/68
2. FLUIDICS
2.1 GENERALITIES
MYTHIC 18 is a fully automated analyzer performing hematological analysis on whole blood.
The human blood venous sample must be collected in an EDTA K3 (Ethylene Diamine Tetracetic Acid, tri
potassic) tube in sufficient quantity. The LMG results are available for six hours after the blood draw. The
use of EDTA K2 leads to a poor quality level of the LMG results.
It must be correctly homogenized before analysis. It is recommended to use a rotary agitator turning
between 20 to 30 turns/mn during 10 minutes.
A volume of insufficient blood for the quantity of anticoagulant or a bad mixing may involve
an erroneous result.
Two dilution rate are performed :
~1/237 to 1/257 for WBC/HB (depend of the lysis quantity, see section 7.2.2.2)
~1/15000 for RBC/PLT
2.2 FLUIDICS SEQUENCES DESCRIPTION :
SAMPLING SEQUENCE :
1- Syringes down : (no valve)
~Less than 9,8 µl of blood sample are draw inside the sampling needle
Diluent prime
Vacuum prime
2- Needle up and syringe up (valve 3+4)
Needle outside cleaning with diluent and waste vacuum
3-Rocker move above the WBC bath
and the syringes up to drain the waste (valve 7)
4- Needle down to the rinsing position
5- Syringe down
WBC bath drain (valve 1)
6- Syringe up
Second outside needle cleaning (valve 4)
Waste syringe drain (valve 7)
7- Syringe down
WBC bath drain (valve 1)
8- Syringe up
Waste syringe drain (valve 7)
WBC DILUTION :
9- Needle down to the dilution position
and the syringes down to prime the diluent (valve 8)
10- Syringe up for the WBC dilution (~2 ml)
Diluent outside of the needle (valve 4)
Diluent inside the needle (valve 4+5)
Bubble to mix the dilution (valve 1)
RBC DILUTION AND LYSING :
11- Syringe down (no valve)
Drawing sample of the first dilution (~20µl)
To Next Page
Loading...
