Cleaning and Sterilization
Maintenance, Cleaning & Troubleshooting
11-9
3 Apply -26 inHg +/- 1 (-12.77 psig +/- .49) vacuum to the empty
sterilizer chamber two times, to remove any residual EO or mois-
ture. Vent the vacuum pump to the outdoors to avoid toxic hazards
to personnel.
4 Insert the equipment to be processed into the gas sterilizer.
5 Heat the chamber and its contents to 54.4 +/- 2.8
o
C (130 +/- 5
o
F).
6 Apply -26 inHg +/- 1 (-12.77 psig +/- .49) vacuum to the sterilizer
chamber.
7 Humidify the chamber at 50% +/- 10% relative humidity for 20 to
30 minutes.
8 Taking a minimum of five minutes, slowly introduce EO sterilant
until the sterilizer unit pressure gauge reaches 11 +/- 1 psig.
Note—At this pressure, the concentration of sterilant in the chamber
will be 600 +/- 50 mg/liter, regardless of the chamber size.
9 Process the equipment to be processed as follows:
Pressure: 11 +/- 1 psig (established in the preceding step).
Time: 2-3 hours
Temperature: 54.4 +/- 2.8
o
C (130 +/- 5
o
F)
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