Thermo Orbitrap Fusion Series - Page 11

Thermo Scientific Orbitrap Fusion Series Hardware Manual xi
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Figure 1. Functional block diagram of the Orbitrap Fusion Series MS . . . . . . . . . . . . . . . . 5
Figure 2. Orbitrap Fusion Series front panel LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Figure 3. Power entry module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Figure 4. Communication connectors (left side of the MS) . . . . . . . . . . . . . . . . . . . . . . . . . 8
Figure 5. Functional block diagram of the vacuum system . . . . . . . . . . . . . . . . . . . . . . . . 16
Figure 6. Schematic of the internal gas supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Figure 7. Gas inlet ports (back of the mass spectrometer) . . . . . . . . . . . . . . . . . . . . . . . . . 18
Figure 8. Placement of the three turbomolecular pumps . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Figure 9. EASY-Max NG API source (H-ESI mode) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Figure 10. API source interface (Orbitrap Fusion MS cross section) . . . . . . . . . . . . . . . . . . 24
Figure 11. Exit lens and RF lens (Orbitrap Fusion and Orbitrap Fusion Lumos
MSs) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Figure 12. Workflow for mass analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Figure 13. Schematic of the Orbitrap Fusion Lumos Tribrid MS ion transmission
path (shown with the ETD source option) . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Figure 14. MP00 RF lens (Orbitrap Fusion and Orbitrap Fusion Lumos MSs) . . . . . . . . . 30
Figure 15. Lens L0 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Figure 16. Multipole MP0 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Figure 17. TK lens (both sides) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Figure 18. Multipole MP1 (Orbitrap Fusion and Orbitrap Fusion Lumos MSs) . . . . . . . . 32
Figure 19. C-trap (Orbitrap Fusion and Orbitrap Fusion Lumos MSs) . . . . . . . . . . . . . . . 33
Figure 20. Ion-routing multipole (IRM) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Figure 21. Multipole MP3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Figure 22. Quadrupole Q1 (side and end views, Orbitrap Fusion and Orbitrap Fusion
Lumos MSs) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Figure 23. Dual-split gate lens (both sides) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Figure 24. Polarity of the RF and DC voltages applied to the Q1 rods . . . . . . . . . . . . . . . . 36
Figure 25. Schematic of the Orbitrap cell and an example stable ion trajectory . . . . . . . . . . 37
Figure 26. Electrodynamic squeezing of ions in the Orbitrap analyzer (r) versus the
increased field strength (z) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Figure 27. Approximate shape of ion packets of different m/z after the voltages
stabilize . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Figure 28. Linear ion trap (LIT) (Orbitrap Fusion MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Figure 29. Assembly for the linear ion trap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Figure 30. Visual representation of the LIT mass analyzer operation (low pressure
cell, Orbitrap Fusion MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Figure 31. Syringe pump setup (top view) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Figures