Waters 2690 - Page 53

Recommended Preparation Procedures 53
Figure 6-4 outlines how to prime, equilibrate, and purge a vacuum degasser-based
Separations Module that has been powered off or is idle with no solvent flow.
Note:
All flow chart references refer to sections in the Waters 2690 Separations Module
Operator’s Guide.
Figure 6-4 Preparing an Idle or Powered-Off Separations Module for Operation
(Degasser-Based Unit)
Set the Degasser to
Continuous
(See “Equilibrating
Solvents in the
Vacuum Degasser”
in Section 4.4.1)
Equilibrate for a
Minimum of 10
Column volumes
(See Section 4.4.5)
Purge the Injector
for a Minimum of 6
Loop Volumes
(See “Purging the
Injector” in
Section 4.4.2)
Perform a Wet Prime
(for One of the
Solvents) for
2
min at
7.500
mL/min
(See Section 4.4.2)
Repeat the Wet
Prime (at the Same
Rate and Time) for
Each Solvent
If You Want to Make
a Gradient Run
(for Example, A:B),
Set the A and B
Composition Fields
in the Status Screen
to
50%
, Then Wet
Prime for
3
min at
5.00
mL/min.
(See Section 4.4.2)
Start
Equilibrate
a
the
Solvent in the
Degasser Chamber
by Setting a Flow
Rate of
0.000
mL/min for
5
min
(See “Equilibrating
Solvents in the
Vacuum Degasser”
in
Section 4.4.1
)
Set the Degasser to
Normal
(See
“Equilibrating
Solvents in the
Vacuum Degasser”
in Section 4.4.1)
a
Except for the short period of time required to equilibrate solvents in the degasser, do not run the degasser
without solvent flow. Refer to the “Degasser Considerations” discussion in Section 1.5 for more details.