106 Error Messages, Diagnostics, and Test Procedures
4. Pump eluent 2 (reservoir C or D) isocratically at 5 mL/min and adjust the recorder
to full scale using the recorder's variable full-scale input.
The recorder is now set to respond so that:
• 0% composition (pure methanol in eluent 1) = 0 percent full scale.
• 100% composition (methanol containing propylparaben in eluent 2) = 100% full
scale.
Entering the Recommended Gradient Table
Enter and save the following gradient table (see the Waters 600E Multisolvent Delivery
System User’s Guide for details about entering a gradient table).
Running the Proportioning Valve Pair Test
1. Proceed to either the Operate Gradient or Operate Methods screen.
2. Press the Start Run function key to execute the gradient table.
3. When the detector baseline is stable at 0%:
• Change the detector sensitivity to 0.1 AUFS and the wavelength to 254 nm.
• Adjust the baseline to 10% full scale by adjusting the zero adjustment of the
recorder.
The recommended settings allow you to easily visualize 1% compositional variances (1%
input changes are set equal to 5% of full scale on the recorder). Therefore, the 10%
Time Flow %A %B %C %D Curve
INITIAL 2.00 100 0 0 0 *
5.00 2.00 90 0 10 0 11
10.00 2.00 100 0 0 0 11
15.00 2.00 90 0 0 10 11
20.00 2.00 0 100 0 0 11
25.00 2.00 0 90 10 0 11
30.00 2.00 0 100 0 0 11
35.00 2.00 0 90 0 10 11
40.00 2.00 0 100 0 0 11
45.00 0.00 0 100 0 0 11
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