8 Making Fluidic Connections to the 600E Pump
described. Microbore techniques especially require care in tubing and connector
installation to ensure optimal chromatographic separation.
• The fittings, connectors, and tubing are chemically resistant to the eluents listed in
the Waters 600E Multisolvent Delivery System User’s Guide, Appendix B, Eluent
Properties.
• Do not nick, kink, or sharply bend the tubing. This may restrict flow. Repeated
bending of tubing will cause tubing failure.
• Subjecting the system to a wide temperature change (for example, when going from
cold-room operation to room-temperature operation) may result in loosened fittings.
Before operating the system at the new temperature, allow the system temperature
to stabilize. When first starting up the system, check all connections for leaks and
retighten fittings as required.
2.1.1 Cutting and Deburring Stainless Steel Tubing
When cutting tubing, avoid angled cuts. These may cause dead-volume formation at the
connection junction due to a poor tubing fit against the connector or part.
Stainless steel tubing with an internal diameter of less than 0.009 inch (0.23 mm) requires
a special tubing cutter. When replacing this tubing, it is recommended to purchase precut
tubing. Refer to Appendix A, Spare Parts.
Cutting Tubing
1. Measure the length of tubing required to connect the components. Allow for slack
so tubing is not pulled tightly around sharp corners.
Note: Use the correct ID tubing when replacing tubing.
2. Use a circular tubing cutter to smoothly cut the tubing to the desired length
(Figure 2-1). Rotate the cutter around the tube until it is cleanly scored.
Caution: Use of solvents that can damage the system could be hazardous
to the operator and voids the 600E system warranty. Refer to the Waters
600E Multisolvent Delivery System User’s Guide, Appendix B, Eluent
Considerations, for eluent compatibility information.
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