Making 600E Pump Connections 17
2.2.2 Setting Up the Sparge System
This section describes how to connect a helium tank to the 600E pump to sparge the eluent
reservoirs. This section covers:
• System sparging overview
• Attaching compression fittings to the helium line
• Connecting the helium sparge supply
Note: The Waters 600E Multisolvent Delivery System Startup Kit includes the
compression screw adaptor fitting, valve, tubing, and other fittings required to
connect the sparge inlet to a 345 KPa minimum to 1035 KPa maximum (50 to 150
psi) helium supply. The 600E system does not include the high-pressure regulator
required to connect the sparge inlet to a helium tank.
System Sparging Overview
Helium sparging reduces the total dissolved gas in the eluent reservoirs and maintains that
condition during operation. Use an ultra-pure-carrier (UPC) grade of helium to prevent
eluent contamination.
The helium disperses through the 600E system in the following route:
1. The helium sparge gas is introduced into the eluent through a diffuser filter that
disperses the helium stream into small gas bubbles.
2. The bubbles increase the effectiveness of the sparge by increasing the surface area
of eluent exposed to helium.
3. The reduction in total dissolved gas occurs as the dissolved gases equilibrate with
the helium at the gas-liquid interface of the bubbles.
4. The displaced gases are carried to the surface and expelled through the bottle vent.
5. Minimizing dissolved gases in the eluents decreases the gas that may be released
when different mobile phases are mixed in the gradient proportioning valve.
For more information on this process, refer to the Waters 600E Multisolvent Delivery
System User's Guide, Appendix B, Eluent Properties. For the procedure on sparging the
reservoirs, refer to the Waters 600E Multisolvent Delivery System User’s Guide, Section
3.1, Sparging the Reservoirs.
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