24 Making Fluidic Connections to the 600E Pump
5. Attach the inlet tubing and outlet tubing to the column as described below
(Figure 2-14). Follow column instructions for flow direction.
• Column Inlet - Pass a piece of 0.009-inch ID tubing from the Rheodyne 7725i
manual injector (or an external autosampler) through the front right port on the
column heater and attach to the column fitting at the rear of the column heater
tray.
• Column Outlet - Pass a piece of 0.009-inch ID tubing through the front left port
on the column heater and attach to the column fitting at the front of the column
heater tray.
6. Pass the column outlet tubing through the notch in the pump drawer front panel. Use
the shortest possible length of 0.009-inch ID tubing from the column outlet to the
detector cell inlet.
7. Check for leaks by pumping eluent through the column. Verify that there are no
leaks at the inlet or outlet connections.
8. Replace the column heater covers.
2.3.3 Installing an RCM 8 x 10
To install an RCM 8 x 10 in the pump slide-out drawer:
1. Turn the latch on the front of the pump (see Figure 2-5) and pull the drawer out.
2. Place the RCM 8 x 10 in the drawer with the cartridge outlet tubing pointing toward
the front of the pump drawer (Figure 2-15). Allow the RCM 8 x 10 to lie flat in the
drawer.
3. Attach the inlet tubing and outlet tubing to the cartridge as follows (see
Figure 2-15).
• Cartridge Inlet – Using a union, attach the 0.009-inch ID tubing from the
Rheodyne 7725i manual injector (or external autosampler) to the cartridge inlet
at the back of the RCM 8 x 10.
• Cartridge Outlet – Using a union, attach a piece of 0.009-inch ID tubing to the
column outlet at the front of the RCM 8 x 10.
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