12 Making Fluidic Connections to the 600E Pump
4. Using a 5/16-inch wrench, tighten the screw an additional 1/4-turn. This seats the
ferrule against the tubing and prevents it from coming off when you disconnect the
tube.
5. Remove the assembled fitting and verify that the length of tubing extending beyond
the ferrule is equal to the length of tubing of other fittings previously installed.
6. Attach the connector.
7. Flow eluent through the new connection at typical operating pressures to verify that
the new connection does not leak. If the connection leaks, tighten it slightly.
Replacing Ferrules
To replace a ferrule, cut off the old ferrule and continue from the start of the ferrule
connection procedure. The compression screw may be reusable.
2.2 Making 600E Pump Connections
This section describes 600E pump setup procedures:
• Setting up the eluent reservoirs
• Setting up the sparge system
STOP
Attention: When the connector is installed in the fitting, ensure the tubing
end seats the receptacle cavity. Gaps will cause excessive dead volume.
STOP
Attention: When testing the connection, be aware of pressure limitations on
other parts of the system, such as columns and detector flow cells.
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