16 Making Fluidic Connections to the 600E Pump
2. Attach a clean diffuser filter to the eluent line.
3. Insert a second tube for the sparge line. Attach a clean diffuser filter to the sparge
line for the eluent reservoir.
4. Repeat steps 1 through 3 for the eluent and sparge tubes of the remaining eluent
reservoirs.
Connecting the Vent Tube to a Fume Hood
When using eluents such as acetonitrile, methanol, or solutions of trifluoroacetic acid,
sparging into open air releases harmful vapors. If working with these or other organic
eluents, run the vent tube from the eluent reservoir caps into an exhaust hood to capture
fumes released at the eluent reservoir.
1. Cut a length of Teflon tubing (from the 600E Startup Kit) sufficient to reach a fume
hood from the eluent reservoir.
2. Push one end of the tubing about one inch into the remaining hole in the reservoir
cap. This tubing is the eluent reservoir vent tube.
3. Connect the other end of the vent tube to a clean diffuser filter, supplied in the
Startup Kit (see Figure 2-7). The diffuser filter provides a slight positive pressure of
sparge gas that inhibits airflow into the reservoir while eluent is being withdrawn.
4. Place the filter end of the vent tube in a fume hood.
5. Insert the eluent reservoir cap into the eluent bottle.
6. Repeat steps 1 through 5 for each eluent reservoir.
Caution: Harmful effects could result from improperly vented eluent or sparge reservoirs.
To avoid respiratory problems, remove vented fumes through a fume hood and proper
ventilation. Use particular care with volatile eluent in a cold room, refrigerator, or other
small enclosed environment.
Caution: Ensure that the end of the vent tubing within the bottle remains
above the level of the eluent. If not, eluent will flow from the bottle through
this vent tube.
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