Pannoramic SCAN II 2.2.0 User’s Guide 5.2.1 Profile Settings panel
• E / Z (Multilayer option): Select Extended focus or Z-stack scanning for the required channels
in accordance with the scanning mode set in the profile.
• D: Deconvolution can activated per channel in this column.
Figure 39 – Filter table
Objective Settings
This field displays the objective used for scanning. Objective type: 20x; 40x
Focus
Focus settings for the scanning. Focus modes can be the following:
• Coarse focus: By selecting the Use manual focus box, you can preset the coarse focus value.
By activating the Coarse focus function, the lower limit value is set to -64, and the upper limit
value is set to +64 of the current focus value.
Note:
Before entering coarse focus value, turn live image on and set a
suitable focus position.
You can accept the current Z-value by clicking Assign Current.
• Interpolated focus: If you select the Interpolated focus option, the coarse focus level is
calculated from three different focus points of the specimen. Carry out a manual focusing
sequence in the preview image on those three points. Set the correct focus value of the
desired preview position by clicking Set in the table of interpolated focus. If you want to go
back to any of the previously set focus points, click the appropriate Goto button.
January 24, 2020 - Rev. 2 3DHISTECH Ltd. 76(156)