Pannoramic SCAN II 2.2.0 User’s Guide 5.2.1 Profile Settings panel
To delete the stored values, click the other proper focus point or turn off the function. The
software calculates a coarse focus level from the three values determined this way as a basis
for the fine focusing routine. You can only define the start parameter here. This will, however,
speed up the focusing process. If you deselect the Use fine focus checkbox, the software
immediately starts scanning on the basis of the interpolated values, without pre-fine-
focusing.
• Auto focus: Focus value is set automatically to match sample characteristics.
• Limit the range: You can define focus limits by selecting Limit the range for focusing. You can
set the upper and lower limits by selecting the desired value in the selection boxes. This is
useful if focusing was not targeted on the specimen, but on a spot on the coverslip.
• Focus frequency : This value determines how many field of views will pass before the sample
is refocused. This value should be selected depending on the unevenness and size of the
specimen.
• Focus Exposure: The exposure time during focusing, it can have three states:
◦ Short
◦ Normal
◦ Long
Sample detection
The Sample detection panel offers options for setting threshold and the selection of scannable areas.
• Source
◦ Brightfield preview – a normal type of illumination in the microscope for scanning the
slide. This option is recommended when scanning in Brightfield mode.
◦ Pre-scan – creates a fast FL preview image that is displayed in the Profile Wizard window.
Scanning process takes a couple of minutes only, and it cannot be interrupted.
◦ Pre-scan high-resolution – creates a high-resolution fast FL preview image of every FOV
within the selected area (in single layer DAPI, with an illumination time of 20ms) that is
displayed in the Profile Wizard window. Scanning process takes a couple of minutes only,
and cannot be interrupted.
January 24, 2020 - Rev. 2 3DHISTECH Ltd. 77(156)
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