Agilent 1260 Infinity RID User Manual 119
Test Functions
8
Refractive Index Calibration
1 Preparation of the sucrose calibration solution.
a To prepare 25 ml of the calibration solution 87.5 mg of the Sucrose
sample is required.
b Add the weighed amount of sample into a suitable volumetric flask.
c Dispense 10 ml of LC grade water into the flask and shake or stir to
dissolve.
d Dilute the contents of the flask to volume with LC grade water.
Wait five minutes and shake again. The solution is now ready for use.
2 Preparing the pump.
a Fill a suitable solvent bottle with LC grade water.
b Connect this bottle to Channel A of the pump, A1 if a binary pump.
3 Using the Agilent LabAdvisor Software (B.01.03 SP4 or later) there are
three screens used for the calibration process.
a RID Module Service Center (via Tools).
b RID Tools screen (via Tools). (If an Agilent pump is part of the system,
the pump section is active.)
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