84 Agilent 1260 Infinity RID User Manual
5 Optimizing the Refractive Index Detector
Refractive Index Detector Optimization
Refractive Index Detector Optimization
Follow these thirteen points to optimize the performance of your refractive
index detector.
1 Position the solvent and waste reservoirs correctly
Position the solvent and waste reservoirs above the level of the refractive
index detector and solvent pump. This maintains a slight pressure in the
sample cell and will improve the performance of the detector.
2 Do not overpressurize the flow cell
Be aware to not exceed a 5 bar pressure drop after the flow cell when
hooking up additional devices like other detectors or a fraction collector. If
an additional detector is installed place upstream in the flow path it before
the G1362A refractive index detector.
3 Use the correct solvents
To minimize baseline noise and drift solvents must be LC grade and filtered
prior to use.
4 Check for leaks
Leaks within the LC instrument that the refractive index detector is
connected to will cause problems with baseline long term noise or drift.
Confirm that the instrument is free from leaks by performing the diagnostic
pressure test (for the high pressure parts of the system between pump and
column). Ensure that the connections from the on-line vacuum degasser to
the pump and the detector inlet, waste and recycle connections are air
tight.
5 Verify frit, filter and fitting quality
Partially blocked frits, filters and fittings can cause baseline long term
noise. Verify that the pressure drop across all such parts is within expected
limits.
6 Control the optical unit temperature
Always control the optical unit temperature (heater = ON) for maximum
detector sensitivity or with samples that could precipitate in the sample
cell at room temperature and set an elevated optical unit temperature at
least 5 °C above ambient conditions.
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