Agilent 1260 Infinity RID User Manual 65
Using the Refractive Index Detector
4
Operation of the Refractive Index Detector
1 Open the purge valve of your pump (by turning it counterclockwise) and set
flow rate to 3 - 5 ml/min.
2 Flush all tubes with at least 30 ml of solvent.
3 Set flow to required value of your application and close the purge valve.
Pump for approximately 30 minutes before starting your application (for some
solvents an even longer flush and equilibration time might be needed).
Tab le 15 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
After an installation Isopropanol Best solvent to flush air out of the
system
When switching between reverse
phase and normal phase (both times)
Isopropanol Best solvent to flush air out of the
system
After an installation Ethanol or Methanol Alternative to Isopropanol (second
choice)if no Isopropanol is available
To clean the system when using
buffers
Bidistilled water Best solvent to re-dissolve buffer
crystals
After a solvent change Bidistilled water Best solvent to re-dissolve buffer
crystals
After the installation of normal phase
seals (P/N 0905-1420)
Hexane + 5 % Isopropanol Good wetting properties
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