86 Agilent 1260 Infinity RID User Manual
5 Optimizing the Refractive Index Detector
Refractive Index Detector Optimization
13 Eliminate mobile phase/column combination problems
Certain mobile phases in combination with specific columns can generate
long term baseline noise. For example acetonitrile/water mobile phases
with certain aminopropyl bonded phase columns. To eliminate the
combination of mobile phase and column as a cause of long term noise
replace the column with Restriction capillary (p/n G1362-87301) and
re-evaluate the detector performance.
Potential Causes for Baseline Problems
Noise (short term)
Typically the sources for short term noise are either electronic (check the
settings for the peak widths, check for ambient sources of electronic noise) or
they are related to the solvents, their composition and flow (in order to verify
this, turn off the pump, consider degassing your solvents, use only premixed
solvents).
Wander (long term noise)
Excessive wander is an indication for a general system or environmental
instability (system or laboratory might not be thermally stable, control
instrument and laboratory temperature). Verify that the solvent properties are
constant over time (flush out contamination, use only stabilized and premixed
solvents). Clean the parts in the flow path and allow the system to be flushed
out and equilibrated.
Drift
Excessive drift is an indication for a general system or environmental
instability (system or laboratory might not be thermally stable, control
instrument and laboratory temperature). Verify that the solvent properties are
constant over time (flush out of contamination, use only stabilized solvents).
Clean parts in the flow path and allow the system to be flushed out and
equilibrated.
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