B-4 Supplied Methods
Touchdown PCR
When the optimal annealing temperature is unknown, one strategy, Touchdown PCR,
incrementally decreases the annealing temperature in early cycles in order to
maximize the yield of specific products.
This supplied method has an initial annealing temperature (65 °C) that incrementally
decreases by an additional 0.5 °C in each of the first 20 cycles, followed by 10 cycles
at 55 °C.
2 Tmp x 102 Tmp x 20
95.0
0:15
65.0
0:30
95.0
0:15
55.0
0:30
*
Start
Return
Method: Touchdown PCR
F1 F2 F3 F4 F5
To Next Page
Loading...
