Applied Biosystems 7500 User Manual

Appendix C
Determining Optimum Dye Concentration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 129
Notes
C
6. In the SDS software, select
InstrumentCalibrate.
7. In the warning dialog box, click to
move the block.
8. In the ROI Inspector, create the ROI image for
each filter, beginning with Filter A:
a. In the Exposure Time field, enter 1024.
b. Click .
c. Select Filter A.
d. Click .
e. Check the image for saturation.
f. Record the coordinate of the well that
displays the brightest possible signal
without saturation. This well contains the
best concentration of the custom pure dye
for Filter A.
Select
Click
a
c
b
No Saturation
Normal
Saturation
Over Saturated
Good Bad

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)