Applied Biosystems 7500 User Manual

Chapter 2 Unpacking and Setting Up the Instrument
Checking the Shipped Materials
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 17
Notes
2
3. Verify that you received the following kits:
Applied Biosystems 7300 or 7500/7500 Fast Real-Time
PCR system Spectral Calibration Kit
7500/7500 Fast
Systems Only
(CY3, CY5, and
TEXAS RED
®
dyes)
7300/7500/7500 Fast Pure Dye Plates
(FAM
™
, JOE
™
, NED
™
, ROX
™
, SYBR
®
Green, TAMRA,
™
and VIC
®
dyes)
Background
Plate
ROI Calibration
Plate
Taq Ma n
®
RNase P
Instrument Verification Plate
Ta qM an
®
RNase P Instrument Verification Plate
Applied Biosystems 7300 or 7500/7500 Fast Real-Time
PCR System Spectral Calibration Kit
7300/7500/7500 Fast Pure Dye Plates
(FAM
™
, JOE
™
, NED
™
, ROX
™
, SYBR
®
Green, TAMRA,
™
and VIC
®
dyes)
Background
Plate
ROI Calibration
Plate
Taq Ma n
®
RNase P
Instrument Verification Plate
✔ Chemistry/Calibration Kits P/N
TaqMan
®
RNase P Chemistry
Verification Plate (7300/7500
systems)
4350584
TaqMan
®
RNase P Chemistry
Fast Verification Plate (7500 Fast
systems)
4351979
The kit(s) appropriate for your
system:
• Applied Biosystems 7300
Real-Time PCR System
Spectral Calibration Kit,
containing:
– Background Plate
– Pure Dye Plates (FAM
™
,
JOE
™
, NED
™
, ROX
™
,
SYBR
®
Green, TAMRA
™
,
and VIC
®
dyes)
– ROI Calibration Plate
4349182
• Applied Biosystems 7500
Real-Time PCR System
Spectral Calibration Kits I and
II, containing:
– Background Plate
– Pure Dye Plates (CY3, CY5,
FAM, JOE, NED, ROX,
SYBR Green, TAMRA,
TEXAS RED
®
, and VIC
dyes)
– ROI Calibration Plate
4349180
(Kit I)
4351151
(Kit II)
• Applied Biosystems 7500 Fast
Real-Time PCR System
Spectral Calibration Kits I and
II, containing:
– Background Plate
– Pure Dye Plates (CY3, CY5,
FAM, JOE, NED, ROX,
SYBR Green, TAMRA,
TEXAS RED, and VIC dyes)
– ROI Calibration Plate
4360788
(Kit I)
4362201
(Kit II)
continued on next page

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)