Applied Biosystems 7500 User Manual

Chapter 3 Installing the Software and Powering On the Instrument
Troubleshooting
34 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Condition: CCD, Filter Wheel, or Shutter Test Failures
1. Power off the instrument, wait 30 seconds, then power on the
instrument.
2. Perform the Instrument Function Test (see page 30).
3. If the test fails again, perform the ROI calibration as explained
in “Performing the Regions of Interest (ROI) Calibration” on
page 35.
4. Determine if the ROI image appears in the ROI Inspector.
Yes – Continue with the installation.
No – Contact Applied Biosystems technical support (see
page xiii) or your service representative.
Condition: Lamp Test Failure
1. Power off the instrument, wait 30 seconds, then power on the
instrument.
2. Perform the Instrument Function Test (see page 30).
3. If the test fails again, select Instrument
Lamp
Status/Replacement.
4. If the Lamp Status/Replacement dialog box reports the lamp
status as Failed, replace the halogen lamp (see “Replacing the
Halogen Lamp” on page 118).
5. Perform the Instrument Function Test (see page 30).
6. If the test fails again, contact Applied Biosystems technical
support (see page xiii) or your service representative.
Condition: Thermal Cycler Test Failure
1. Power off the instrument, wait 30 seconds, then power on the
instrument.
2. Perform the Instrument Function Test (see page 30).
3. If the test fails again, contact Applied Biosystems technical
support (see page xiii) or your service representative.
Troubleshooting – Function Tests (continued)
Lamp
status

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)