Applied Biosystems 7500 User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Overview
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 51
Notes
5
The software then uses the calibration file during subsequent runs to remove the
background fluorescence from the run data.
Background
Fluorescence
Fluorescence data collected by the Applied Biosystems 7300/7500/7500 Fast Real-Time
PCR System includes a fluorescent signal inherent to the system, commonly referred to
as background fluorescence. Background fluorescence is a composite signal found in all
spectral data. This signal consists of fluorescence from several sources, including:
• Background electronic signal
• Contaminants in the sample block
• The plastic consumable (plates and caps)
Guidelines for
Calibration
For a new instrument:
• Always start with a new calibration kit.
• Make sure the centrifuge you use is clean. Before centrifuging, wipe down the
bucket using a tissue.
• Handle the calibration plates with care to prevent contamination. Do not place
plates on a lab bench, which may contaminate the plate. Always put calibration
plates back into their original bags.

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)