Applied Biosystems 7500 User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Performing the Optical Calibration for the 7500/7500 Fast System
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 63
Notes
5
Analyzing the Optical Calibration Data
1. In the SDS software, click (or select AnalysisExtract).
The software extracts the optical calibration, then displays a message indicating the
extraction is complete.
2. Click .
3. In the SDS software, select FileClose.
Unloading the
Plate
PHYSICAL INJURY HAZARD. During instrument operation, the
sample block can be heated to 100 °C. Before performing the following procedure, be
sure to wait until the sample block reaches room temperature.
1. Remove the ROI calibration plate:
a. Press the tray to open it.
b. Remove the ROI calibration plate.
c. Press the tray to move it into the instrument.
2. Place the ROI calibration
plate inside its packaging
sleeve, then return it to the
spectral calibration kit in
the freezer.
IMPORTANT! Do not discard the ROI calibration plate. If you store the plate in its
original packaging sleeve, you can use the plate up to three times after you open it.
Continue with “Performing the Pure Dye Calibration” on page 67.
-20 to -

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)